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小鼠沉默調節蛋白2(SIRT2)酶聯免疫吸附檢測試劑盒

ELK9507

規格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Mouse SIRT2(Sirtuin 2) ELISA Kit
Reactivity:	Mouse
Alternative Names:	SIR2L; SIR2-L; SIR2L2; Silent Information Regulator 2 Protein; NAD-Dependent Deacetylase Sirtuin-2; Silent Mating Type Information Regulation 2 Homolog
Assay Type:	Sandwich
Sensitivity:	0.68 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Developmental science;
Uniprot ID:	Q8VDQ8
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SIRT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SIRT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOLOGICAL TRACE ELEMENT RESEARCH

Anti-Obesity Effects of Calcium Fructoborate by Inhibiting Adipogenesis and Increasing SIRT’s Expression in 3T3-L1 Cells

日期：2025-01-17 IF：3.4

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.096	2.003
50.00	1.607	1.514
25.00	1.211	1.118
12.50	0.813	0.720
6.25	0.538	0.445
3.13	0.335	0.242
1.57	0.252	0.159
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SIRT2 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	81-97%	89%
Heparin plasma(n=5)	84-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.