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大鼠孕酮(PG)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8385

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat PG(Progesterone) ELISA Kit
Reactivity:	Rat
Alternative Names:	P4; Pregn-4-Ene-3,20-Dione
Assay Type:	Competitive Inhibition
Sensitivity:	0.42 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PG in the samples is then determined by comparing the OD of the samples to the standard curve.

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標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	0.217
50.00	0.386
25.00	0.524
12.50	0.823
6.25	1.168
3.13	1.557
1.57	1.796
0.00	2.213

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PG and the recovery rates were calculated by comparing the measured value to the expected amount of PG in samples.

Matrix	Recovery range	Average
serum(n=5)	78-93%	85%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	90-103%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.