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小鼠Ki-67蛋白(Ki67P)酶聯免疫吸附檢測試劑盒

ELK6495

規格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Mouse Ki67P(Ki-67 Protein) ELISA Kit
Reactivity:	Mouse
Alternative Names:	MKI67; KIA; Ki-67; Antigen Identified By Monoclonal Antibody Ki-67
Assay Type:	Sandwich
Sensitivity:	0.127 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Reproductive science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ki67P. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ki67P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ki67P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ki67P in the samples is then determined by comparing the OD of the samples to the standard curve.

PHYTOMEDICINE

Xiyangshen Sanqi Danshen granules attenuated D-gal-induced C57BL/6J mouse aging through the AMPK/SIRT1 signaling pathway

日期：2025-01-17 IF：6.7

標準曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.172	2.099
10.00	1.647	1.574
5.00	1.174	1.101
2.50	0.726	0.653
1.25	0.556	0.483
0.63	0.262	0.189
0.32	0.156	0.083
0.00	0.073	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Ki67P and the recovery rates were calculated by comparing the measured value to the expected amount of Ki67P in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	92-105%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ki67P and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.