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大鼠雌激素受體α(ERa)酶聯免疫吸附檢測試劑盒

ELK5627

規格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Rat ERa(Estrogen Receptor Alpha) ELISA Kit
Reactivity:	Rat
Alternative Names:	ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1
Assay Type:	Sandwich
Sensitivity:	0.131 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;
Uniprot ID:	Q5QJV7
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ERa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ERa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ERa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ERa in the samples is then determined by comparing the OD of the samples to the standard curve.

3 Biotech

Methanolic extract of wheatgrass (Triticum aestivum L.) prevents BPA-induced disruptions in the ovarian steroidogenic pathway and alleviates uterine inflammation in Wistar rats

日期：2025-01-17 IF：2.6

標準曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.165	2.079
10.00	1.763	1.677
5.00	1.174	1.088
2.50	0.811	0.725
1.25	0.508	0.422
0.63	0.423	0.337
0.32	0.232	0.146
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ERa and the recovery rates were calculated by comparing the measured value to the expected amount of ERa in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.