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小鼠AMP激活蛋白激酶α1(PRKAa1)酶聯免疫吸附檢測試劑盒

ELK5105

規格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Mouse PRKAa1(Protein Kinase, AMP Activated Alpha 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
Assay Type:	Sandwich
Sensitivity:	0.66 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;
Uniprot ID:	Q5EG47
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRKAa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRKAa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRKAa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRKAa1 in the samples is then determined by comparing the OD of the samples to the standard curve.

PHYTOMEDICINE

Xiyangshen Sanqi Danshen granules attenuated D-gal-induced C57BL/6J mouse aging through the AMPK/SIRT1 signaling pathway

日期：2025-01-17 IF：6.7

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	1.997	1.904
50.00	1.621	1.528
25.00	1.201	1.108
12.50	0.880	0.787
6.25	0.586	0.493
3.13	0.340	0.247
1.57	0.186	0.093
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PRKAa1 and the recovery rates were calculated by comparing the measured value to the expected amount of PRKAa1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-95%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRKAa1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.