制服丝袜在线视频香蕉,欧美日韩国产精品久久电影,欧洲亚洲欧美国产日本高清

^{<table id="u10bl"></table>}

產品中心

人核因子κB亞基p105(NFKB-p105)酶聯免疫吸附檢測試劑盒

ELK2228

規格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
Reactivity:	Human
Alternative Names:	NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	P19838
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Oncology

Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines

日期：2025-01-17 IF：3.5
Frontiers in Oncology

Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines

日期：2025-01-17 IF：3.5
Frontiers in Oncology

Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines

日期：2025-01-17 IF：3.5
Frontiers in Oncology

Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines

日期：2025-01-17 IF：3.5
Kafkas Universitesi Veteriner Fakultesi Dergisi

Morphological and Molecular Changes in Renal Tissue Following Experimental Unilateral Ureteral Obstruction in Rat Kidneys

日期：2025-01-17 IF：0.8

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.135	2.052
5.00	1.562	1.479
2.50	1.121	1.038
1.25	0.826	0.743
0.63	0.523	0.440
0.32	0.312	0.229
0.16	0.156	0.073
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.