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人基質金屬蛋白酶11(MMP11)酶聯免疫吸附檢測試劑盒

ELK2113

規格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human MMP11(Matrix Metalloproteinase 11) ELISA Kit
Reactivity:	Human
Alternative Names:	SL3; ST3; STMY3; Stromelysin 3
Assay Type:	Sandwich
Sensitivity:	0.27 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Infection immunity;
Uniprot ID:	P24347
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP11 in the samples is then determined by comparing the OD of the samples to the standard curve.

Nature Communications

Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients

日期：2025-01-17 IF：14.7

標準曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	2.075	1.986
25.00	1.502	1.413
12.50	1.174	1.085
6.25	0.963	0.874
3.13	0.492	0.403
1.57	0.385	0.296
0.79	0.168	0.079
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MMP11 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP11 in samples.

Matrix	Recovery range	Average
serum(n=5)	77-95%	86%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP11 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.