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一步法人血管內皮鈣黏蛋白(VE-Cadherin)酶聯免疫吸附檢測試劑盒

ELK088ES

規格：	價格：
64T	￥1960.00
112T	￥2800.00

下載說明 ①

Overview

Product name:	EasyStep Human VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Reactivity:	Human
Alternative Names:	CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type:	Sandwich
Sensitivity:	32.1 ng/mL
Standard:	10 μg/mL
Detection Range:	0.16-10 μg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (μg/mL)	OD	Corrected OD
10.00	2.075	1.987
5.00	1.608	1.520
2.50	1.205	1.117
1.25	0.852	0.764
0.63	0.514	0.426
0.32	0.317	0.229
0.16	0.252	0.164
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	89-103%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.