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一步法人表皮生長因子受體2(EGFR2)酶聯免疫吸附檢測試劑盒

ELK078ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit
Reactivity:	Human
Alternative Names:	CD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
Assay Type:	Sandwich
Sensitivity:	16.9 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;Tumor immunity;Reproductive science;Hepatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2, and the Human EGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR2. After TMB substrate solution is added, only those wells that contain Human EGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.169	2.086
2500.00	1.647	1.564
1250.00	1.116	1.033
625.00	0.641	0.558
312.50	0.496	0.413
156.25	0.388	0.305
78.13	0.244	0.161
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EGFR2 and the recovery rates were calculated by comparing the measured value to the expected amount of EGFR2 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-90%	84%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.