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一步法人干擾素γ(IFNg)酶聯免疫吸附檢測試劑盒

ELK067ES

規格：	價格：
64T	￥1960.00
112T	￥2800.00

下載說明 ①

Overview

Product name:	EasyStep Human IFNg(Interferon Gamma) ELISA Kit
Reactivity:	Human
Alternative Names:	IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Assay Type:	Sandwich
Sensitivity:	15 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, cell
Assay Length:	1.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.038	1.930
500.00	1.521	1.413
250.00	1.186	1.078
125.00	0.892	0.784
62.50	0.447	0.339
31.25	0.481	0.373
15.63	0.226	0.118
0.00	0.108	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.